• L I G H T W I N D
  • SOLUTIONS
    • Semiconductor >
      • Thin Film Measurement
      • Plasma and CVD Gas Analysis >
        • L Series: Downstream Process Spectroscopy
        • S Series: Window Mount
      • Plasma and Wet Etch Process Endpoint >
        • Wet Etch Endpoint
    • Materials Analysis >
      • Reflectance
    • Chemical Analysis >
      • Gas Measurement
      • Absorption Spectroscopy
      • Fluorescence Spectroscopy
      • Raman >
        • Raman Products >
          • Portable Raman
          • PreConfigured Raman Systems
          • Automated Raman Systems
          • Raman Software
    • MultiSpectral Imaging >
      • Multispectral Example
      • Multispectral Imaging Resources
    • Education
    • Solar
    • BioScience
    • Food Science >
      • Food Science Resources
      • Comparison Of F Series Analyzers
    • Plasma Spectroscopy >
      • Plasma Spectroscopy Kits
      • Plasma Spectroscopy Systems
  • PRODUCTS
    • Spectrometers, Detectors, Fiber Optics
    • Fiber Optic Cables
    • Flow Cells
    • Lasers
    • Software >
      • Analyze IQ
      • Ocean Optics Software
  • SERVICES
    • OEM Components
    • Custom Engineering
  • RESOURCES
    • Lightwind Software Instructional Videos >
      • Configuring and Viewing Data
      • Template and Recipe Operations
      • Endpoint
  • CONTACT
    • ABOUT US
  • Category
  • Solara

FLUORESCENCE IMAGING

Photo courtesy of University of Muenster Institute of Inorganic and Analytical Chemistry  

​Using either the presence of natural fluorescence (autofluorescence), fluorescent proteins in biological samples or more commonly by the use of fluorescent dyes to stain the sample prior to viewing, imaging a sample using fluorescence can greatly enhance the resolution over other microscopy methods. It is widely used in life sciences to image cell or protein structures, microtitre plate analysis and high throughput screening analysis. Fluorescence Lifetime IMaging spectroscopy(FLIM) uses similar principles to monitor fluorescence decay rather than intensity benefiting from reduced scatter interference.
Cyanine, Atto and azo dyes  amongst others are excited by laser light and emit light of a longer wavelength allowing fine structures to be made visible below the diffusion limited resolution of standard confocal microscopy. The vast array of available dyes allows highly specific structures to be imaged when partnered with excitation light of the right wavelength. This has traditionally been dominated by diode lasers, due to the wider array of wavelengths available with this technology, but as the technology of DPSS lasers improves, wavelengths well matched to the application are now coming on to the market which offer greatly improved stability in power and wavelength, as well as a far narrower linewidth, reducing the requirement for expensive filter sets and allowing imaging of fluorescence wavelengths far closer to the excitation wavelength.
With required power in the order of milliwatts, compatibility and ease of use becomes important, together with the availability across the wavelength spectrum and spectral stability. Many of our lasers are suited to this area due to their stability, size and easy integration into any microscope system, including the use of fiber coupling and all can be controlled by software or manually through software control of the laser power supply.
RELATED PRODUCTS
Tau Laser System
​tau -  Small and easily integrated
​

               532       671 nm  

More information
Picture
gem-    for OEM integration 
​

473        532     561      
660     671nm. 
More information
Picture
ventus - for scientific research applications ​
 473       532        561       660         671nm 
More information
CUVETTE HOLDER FLUORESCENCE
CUV FL Cuvette Holder
QPOD Temperature controlled cuvette holder for absorption and fluorescence
QPOD for Temperature Control
Cuvette holder for absorption and fluorescence
Four way Cuvette Holder

APPLICATION INFORMATION

​Title and Size
​

Scientific paper - A scanning nano-spin ensemble microscope        2204KB
Article - Magnetic Field Imaging                                                               1574KB
Article - Single Molecule Fluorescence                                                  1964KB
Scientific paper - Modelling of Photochemical Reactions                     234KB

Simple Illustration of Laser Fluorescence vs Wavelength

Picture
Copyright © 2020
CONTACT US
  • L I G H T W I N D
  • SOLUTIONS
    • Semiconductor >
      • Thin Film Measurement
      • Plasma and CVD Gas Analysis >
        • L Series: Downstream Process Spectroscopy
        • S Series: Window Mount
      • Plasma and Wet Etch Process Endpoint >
        • Wet Etch Endpoint
    • Materials Analysis >
      • Reflectance
    • Chemical Analysis >
      • Gas Measurement
      • Absorption Spectroscopy
      • Fluorescence Spectroscopy
      • Raman >
        • Raman Products >
          • Portable Raman
          • PreConfigured Raman Systems
          • Automated Raman Systems
          • Raman Software
    • MultiSpectral Imaging >
      • Multispectral Example
      • Multispectral Imaging Resources
    • Education
    • Solar
    • BioScience
    • Food Science >
      • Food Science Resources
      • Comparison Of F Series Analyzers
    • Plasma Spectroscopy >
      • Plasma Spectroscopy Kits
      • Plasma Spectroscopy Systems
  • PRODUCTS
    • Spectrometers, Detectors, Fiber Optics
    • Fiber Optic Cables
    • Flow Cells
    • Lasers
    • Software >
      • Analyze IQ
      • Ocean Optics Software
  • SERVICES
    • OEM Components
    • Custom Engineering
  • RESOURCES
    • Lightwind Software Instructional Videos >
      • Configuring and Viewing Data
      • Template and Recipe Operations
      • Endpoint
  • CONTACT
    • ABOUT US
  • Category
  • Solara